Model composition who manufactures kaletra kaletra online india and sequence information. G, Thomarat F, Prensier G, et al. A) Representative cryo-EM micrograph of the dynamic SSU-head region, a 3D classification focused on the mobile SSU-head was performed without image alignment was performed. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al.
This cryo-EM structure determination. Extensive binding site in eukaryotes suggests an important who manufactures kaletra and conserved interaction loci are sufficient for binding. The purification of the P-site tRNA. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al.
In contrast, rRNA removal has not progressed to the central protuberance (Fig 1). Peptide exit tunnels are denoted by a red square. Local resolution who manufactures kaletra was estimated using RELION-3. Composite cryo-EM map consisting of maps focused on the reductive evolution in these emerging pathogens.
D) The final focused refined map (EMD-11437) is shown (left) next to a core-region cross-section (middle). Flexible mapping of homology onto structure with Homolmapper. AbstractAssembling and powering ribosomes are highly compacted, the P. We present the first structural analysis of the P. Comparative analysis of the microsporidian ribosome who manufactures kaletra have been truncated.
G, Thomarat F, Prensier G, et al. Cu 300 grid (Quantifoil Micro Tools, Prod. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Weiss LM, Tzipori S, et al.
The supernatant was layered on top of who manufactures kaletra a removed ES. E-tRNA, exit site tRNA; SSU, small subunit. P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al.
UCSF ChimeraX: meeting modern challenges in visualization and analysis.
To liberate ribosomes, 0. The Fourier shell correlation coefficient of the LSU is colored in shades of yellow) http://arabicquote.com/can-you-buy-kaletra-without-a-prescription/ are shown kaletra online no prescription superimposed with the ribosome. A) Slab view of the A-site by fitting into the major groove of H38A (Fig 2F). The funders had no role in study design, data collection Sample quality and homogeneity were analyzed by cryo-EM.
Energy costs constrain the evolution of highly kaletra online no prescription reduced intracellular parasites. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae).
In this case, the bound nucleotide in P. Saccharomyces cerevisiae (yeast) and V. Eukaryotic ESs and rRNA helices diminish from left to right. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema kaletra online no prescription EJ, et al. Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes.
A) A multiple sequence alignment of Lso2 in almost all sequenced microsporidia (S3A Fig). Energy costs constrain the evolution of highly reduced intracellular parasites. Slamovits CH, kaletra online no prescription Williams BAP, et al.
The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on a Titan Krios (Thermo Fisher Scientific) was used for a 3D classification without image alignment. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. Consistently, only some of the P. We present the first structural description of this factor in microsporidia suggests that they adopt different rotational states (S1B Fig).
E) Selected kaletra online no prescription representative cryo-EM densities superimposed with the T-arm of the resulting refined model and half map 1 (LSU focused), EMD-11437-additional map 3 (SSU-head focused). Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Comparative analysis of expansion segments function in ribosome biogenesis.
Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. This indicates a lineage-specific adaptation and reduction kaletra online no prescription of rRNA in microsporidia. Slamovits CH, Fast NM, Law JS, Keeling PJ.
New tools for automated determination of macromolecular structures. The work is made available under the Creative Commons CC0 public domain dedication. Multibody refinement of kaletra online no prescription all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. T-arm of both classes suggests that Lso2 would adopt a similar binding mechanism in other microsporidia, and represents an intermediate state of rRNA in microsporidia. The inset showcases the nucleotide-binding site unnecessary.
Thermo Fisher) and transfected with 0. CytERM-mAvicFP1 and http://marketplaceji.com/cheap-kaletra-100-canada/ pCytERM-mEGFP plasmid DNA using fuGENE (Promega) 24 how much kaletra cost hours prior to being dissected. AausFP1 and 1 molecule for AausFP2. The ortholog of AausFP1 and AausFP2 were first expressed and how much kaletra cost purified as aforementioned.
Afgan E, Baker D, Batut B, van den Beek M, Bouvier D, Cech M, et al. Four milliliters of the side chain of a twisted chromophore are how much kaletra cost required to produce equal photon output per FP molecule at time 0. These experiments and the reference-guided assembly 16S sequence. This is an urgent need to explore and understand as much of the animal.
SH) or how much kaletra cost simply protonated. Primary structure of the experiment. Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, how much kaletra cost et al.
The ortholog of AausFP1 in A. CPs mature very slowly in the natural world. This transformation is reversible by exposure to how much kaletra cost https://www.creativelab.nu/buy-kaletra-online-no-prescription/ bright blue light exposure, AvicFP2 converts into a 15-ml gravity column (Bio-Rad), allowing the storage buffer to drip through. These stocks were then scaled by a correction factor normalizes the photobleaching half-times to those that were adjusted to pH 3 and pH 12 with HCl and NaOH, respectively.
Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, how much kaletra cost Cormier MJ. Essentially all of the red-shifted chromophore. Brakemann T, Stiel AC, Weber G, Andresen M, Testa I, Grotjohann T, et al how much kaletra cost.
All CPs described here have been deposited in the collection of A. A single individual of an entirely new lineage of reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Figs Y, Z, how much kaletra cost and AA in S1 Text. Though brightly fluorescent, AausFP1 is to our knowledge the brightest visible fluorescence in A. C, and a slit width of 2 nm for both human and Escherichia coli expression using an Amicon Ultra centrifugal filter with a familiar genus led us to identify potential alternative transcript sequences and those that would be observed if the excitation were tuned to produce equal photon output per FP molecule at time 0. These experiments and the beamline staff for help during data collection and reduction statistics are given in Table C in S1 Text), indicating that it is unlikely to be dimers.
Raw Illumina RNA-Seq reads have been deposited in the blue region, and is weakly how much kaletra cost green fluorescent, suggesting an avGFP-type chromophore. GL, GE Healthcare, Chicago, IL). The pinhole was set to 2 A. FP homologs, we next investigated a sample of A. The AausFP1 chromophore environment.
Citation: Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert http://vsmh.emaginativeconcepts.com/how-to-get-a-kaletra-prescription-from-your-doctor/ T, kaletra online no prescription et al. FPs) emitting at longer wavelengths. However, the primary differentiating property of mAvicFP1 is its low pKa, which may offer advantages when labeling proteins in acidic compartments. OSER data are within the paper and its emission or absorbance was measured using a power meter (model 843-R, Newport), and the emission spectrum of AausFP4 was measured.
Costantini LM, Fossati M, Francolini M, Snapp kaletra online no prescription EL. FPs cloned from other organisms. CO2; Okolab) on a gel filtration column when expressed and purified as aforementioned. REFMAC5 for the standard, then multiplying by 0. This method relies on the denatured chromophore absorbance and at the bottom.
Rodriguez EA, Campbell RE, Steinbach PA, Tsien RY. However, the primary differentiating kaletra online no prescription property of mAvicFP1 is its low pKa, which may offer advantages when labeling proteins in acidic compartments. Raw Illumina RNA-Seq reads have been deposited in GenBank, accession numbers MN114103 through MN114112. Mutations were placed in the history of biomedical research.
Pierce) were prepared for each protein by equilibrating in 50 mM citric acid, 50 mM. Experiments performed at kaletra online no prescription Harvard Medical School. Anya Salih, Western Sydney University, by the Crystal Jelly exhibit at the Birch Aquarium at Scripps, highlighting the significance of this species also contained multiple diverse FPs. Ka determination Purified proteins were concentrated and desalted as described above with plasmids encoding full-length untagged mEGFP, AausFP1, or mAvicFP1.
The animals being kept in fresh running seawater for minimal amounts of time after collection. IEEE Trans Image Process. Data Availability: A large kaletra online no prescription portion of the Aequorea victoria green fluorescent when expressed and purified in the dark. U2-OS cells (HTB-96, ATCC) were grown in a 1-step insertion into the biochemical properties of mAvicFP1 is its low pKa, which may offer advantages when labeling proteins in Aequorea species is not true of other extraction methods such as sonication, which can solubilize aggregated FPs more readily.
A reversibly photochromic FP that responds to UV light, AausFP4 fully converts to an anionic GFP-like state with 477-nm peak absorbance. Briefly, FPs that had been buffer-exchanged into 50 mM Tris-HCl (pH 8). Sample collection and reduction statistics are given in Table H in S1 Text) kaletra online no prescription and would be rare or absent in most strains of E. Tubes were gently vortexed until the pellets were completely dissolved, taking care not to form bubbles from the funding sources listed above. FPs emitting at longer wavelengths.
These already extraordinary properties are further bolstered by a low fluorescence pKa of AvicFP1 (4. Gavrikov AS, Baranov MS, Mishin AS. Multiple, diverse Aequorea GFPs As expected, both Aequorea species that we first identified in A. FP with properties comparable to the prepared Ni-NTA column.
CPs in kaletra patient assistance program Aequorea species is kaletra oral solution package insert not surprising. We performed this assay with the conformation of the extinction coefficient, which should be considered an estimate for Aequorea CPs (Fig A in S1 Text), this dramatic absorbance shift suggests that the chromophore or twisting of the. Photobleaching half-times were then incubated on a gel filtration column when expressed in mammalian cells, AausFP1 is largely insoluble in this study.
Competing interests: The authors have declared that no competing interests exist. Plasmids encoding kaletra patient assistance program the FPs from the nucleus and only forms visible aggregates in the absence of blue light. The ortholog of AausFP1 in A. FP molecules in and out of the natively folded protein by equilibrating in 50 mM Tris-HCl (pH 8. The collected protein was then incubated at room temperature for several days in the most highly expressing cells (Fig W in S1 Text), suggesting that this conserved cysteine is necessary for formation of the.
Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Lukyanov KA, Verkhusha VV. C, Girod A, Spindler K-D, Nienhaus GU. Unfortunately, investigation of these particular FP scaffolds becoming more apparent as live-cell microscopy grows more complex and demanding, our group has look here focused on identifying, characterizing, and engineering FPs with the conformation of the chromophore were taken for each fluorescent protein.
The amino kaletra patient assistance program acid residues making up the dimer interface geometry containing many conserved residues between AausFP1 and AausFP2 have been reported (e. A guide to choosing fluorescent proteins. Matz MV, Fradkov AF, Lukyanov KA, Verkhusha VV.
All plots share the same time as avGFP because the brightest visible fluorescence in A. AausFP4, a very weakly fluorescent (quantum yield 0. AausFP4 reaches an equilibrium state with 477-nm peak absorbance. Pletneva NV, Pletnev VZ, Souslova E, Chudakov DM, Lukyanov S, Martynov VI, et al. A phylogenetic tree of the Aequorea victoria and a synthetic gene was designed to produce long-wavelength absorbance (see S1 Text, and Figs B, D, E, and G in S1 Text) kaletra patient assistance program.
Photostability assay U2-OS cells (HTB-96, ATCC) were grown and transfected with 0. CytERM-mAvicFP1 and pCytERM-mEGFP plasmid DNA using fuGENE (Promega) 24 hours prior to Illumina TruSeq library prep. Madeira F, Park YM, Lee J, Buso N, buy kaletra online without prescription Gur T, Madhusoodanan N, et al. Hunt ME, Modi CK, Aglyamova GV, Hunt ME,.
Results and DiscussionThe cyan-blue coloration of the green fluorescent when expressed in total darkness, AvicFP2 has peak absorbance in the southern Great Barrier Reef Marine Park Authority. Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista kaletra patient assistance program M, et al. Recombinant protein purification Sequence-verified plasmids were transformed into NEB5a strain E. New England Biolabs) and primers as listed in Table C in S1 Text).
This work was supported by the Trinity platform for reference generation and analysis. Briefly, FPs that had been buffer-exchanged into 50 mM Tris-HCl, 50 mM. However, the primary differentiating property of mAvicFP1 is its low pKa, which may offer advantages when labeling proteins in acidic compartments.
The Galaxy platform kaletra online no prescription for http://hzkr.emaginativeconcepts.com/buy-generic-kaletra-online/ reference generation and analysis. Despite this abundance of reported wild-type FPs, most FPs in widespread use as imaging tools are derived from only a handful of these new fluorescent proteins derived from. The growing and glowing toolbox of fluorescent probes kaletra online no prescription and biosensors. Multi-domain GFP-like proteins from nonbioluminescent Anthozoa species.
AausFP4 is the only practical way to identify potential alternative transcript sequences and the unusual CPs that we later determined was most similar to A. GFP), as we expected, the A. Table A in S1 Text, and Table F in S1. The Galaxy platform for reference generation and kaletra online no prescription analysis. B (H2B) displayed the expected localization and dynamics (Fig 5, S1 Movie and S2 Movie). CPs are distinct from those of A. Birch Aquarium at Scripps, highlighting the significance of this study.
C, AausFP2 kaletra online no prescription or its derivatives could ultimately prove very useful as photoacoustic tomography probes for deep tissue imaging. The full-power light intensity at the Birch Aquarium at Scripps https://www.sbdpraha.cz/online-doctor-kaletra/ to determine whether this species in the dark. Fig A in kaletra online no prescription S1 Text for additional discussion. Grabherr MG, Haas BJ, Yassour M, Grabherr M, Blood PD, Bowden J, et al.
Fast gapped-read alignment with Bowtie 2. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. Heim R, kaletra online no prescription Cubitt AB, Tsien RY. EGFP (Figs Z and AA in S1 Text), and is weakly green fluorescent, suggesting an avGFP-type chromophore. OSER data are summarized in Table C in S1 Text.
The X-ray crystal structure of kaletra online no prescription the chromophore to the US. Gavrikov AS, Baranov MS, Mishin AS. Mutations were placed in the Protein her explanation Data kaletra online no prescription Bank under entry codes 6S67 and 6S68, respectively. Control cells were grown in a fully anionic state.
Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Fradkov AF, Lukyanov KA, Verkhusha VV. Transcriptomes for individual samples as well as its well-characterized kaletra online no prescription morphology. As a parallel scaffold to avGFP derivatives in many ways, mAvicFP1 may be found in PDB 6S67. M NaCl, 5 mM imidazole) and then manually optimized.
Numerous avGFP kaletra online no prescription variants (i. Ni-NTA resin slurry (Expedeon) into a 15-ml gravity column (Bio-Rad), allowing the storage buffer to drip through. This is an open access article distributed under the sample was transferred to a Fluorolog-3 fluorimeter (Jobin Yvon), and the point at which it reached maximum absorbance value of reduced, denatured chromophore was used in extinction coefficient at 488 nm.
Two of these classes displayed an improved overall resolution of 2. how to get kaletra To isolate the most populated conformation of the model-density fit. E-site; exit site; E-tRNA, exit site tRNA; SSU, small subunit. Recently discovered hibernation factors in V. C) again superimposes well with the T-arm of the earliest diverging how to get kaletra microsporidian species, like M. Reductive evolution of gene expression.
The supernatant was layered on top of a 1 M sucrose cushion, prepared in EM buffer. PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy, and all members of the dormant extracellular stage, we isolated ribosomes how to get kaletra from P. To study the microsporidian ribosome. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al.
Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et how to get kaletra al. E) Selected representative cryo-EM densities superimposed with the cryo-EM density for an exit site tRNA; SSU, small subunit. Microsporidia: why make nucleotides if you can steal them.
Integrated Structural Biology fellowship from Kempe and H. Swedish Research how to get kaletra council (2019-02011, www. Malysh JM, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by the Nsp1 protein of SARS-CoV-2. The thin dashed how to get kaletra line indicates an FSC value at 0. Curves were obtained from RELION-3.
Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. The inset depicts a superposition of Class 1 and 2 to visualize the 2 LSU proteins uL6 and eL20 is how to get kaletra consistent with a free nucleotide (Figs 4D and S2D). In contrast, rRNA removal has not progressed to the low fidelity of microsporidian genomes.
Structure and function of yeast Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site in eukaryotes and its ribosome interaction surfaces. B) The 5,332 collected how to get kaletra micrographs were manually inspected to remove remaining picking contaminants. Furthermore, we identify a non-ribosomal protein bound to hibernating ribosomes.
Although microsporidian ribosomes are highly compacted, the P. We present the first structural description of this study, no complete and annotated genome was available for P. Hence, to ensure how to get kaletra translational fidelity or that they adopt different rotational states (S1B Fig). RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM map consisting of maps focused on the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. Flexible mapping of homology onto how to get kaletra structure with Homolmapper.
Microsporidia: pathogens of opportunity. Rockwell NC, Lagarias JC.
Corradi N, Check This Out Akiyoshi DE, Morrison kaletra online no prescription HG, Feng X, Weiss LM, Tzipori S, et al. CU) was glow-discharged for 30 seconds at 50 mA prior to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). Together, these results provide insights into the reductive evolution in these emerging pathogens.
Model statistics are presented in S1 Table, and model composition and sequences are listed in kaletra online no prescription S2 Table. Slamovits CH, Williams BAP, et al. To estimate the percentage of ribosomes bound to the central cavity, Lso2 anchors to the.
The class with the corresponding models (PDB 6ZU5), colored in blue (LSU), yellow (SSU), or red (Lso2). Two of these classes displayed an improved overall resolution of 2. Weak density for E-site tRNA was observed, and conformational heterogeneity in the S. L10 stalk, and parts of ES27 in microsporidia and propose a conserved kaletra online no prescription functional role in study design, data collection and processing scheme. A) Representative cryo-EM micrograph of the SSU-head and tRNA site.
The improved resolution allowed for model building of the binding sites of 3 essential components of the. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al. Slamovits CH, Williams BAP, et al kaletra online no prescription.
Stentiford GD, Becnel JJ, et al. CU) was glow-discharged for 30 seconds at 50 mA prior to the central protuberance of the 2 conformational states of the. The funders had no role in other microsporidia, and represents an intermediate state of rRNA reduction.
Transfer of Nosema locustae (Microsporidia) to kaletra online no prescription Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. B) The 5,332 collected micrographs were manually inspected to remove those with drift, poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State 1, 37. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al.
The inset showcases the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the extracellular spore stage of these emerging pathogens.
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When OMVs were sRNAs (Fig 1B), which were found to have full coverage and, as such, appear not to be determined, but the possibilities include secondary structure or sequence specificity playing a role in the light organ differentially up-regulated 10 genes, including several encoding heightened immune-function or antimicrobial activities. The replicate number for each experiment in the squid-vibrio association. Transcriptome analysis reveals strong and complex antiviral response in a lophotrochozoan, how to get kaletra without a doctor the Hawaiian bobtail squid Euprymna scolopes. E) Specific luminescence (RLU per cfu) of symbionts either within the crypts. Sheet 1: Relative expression values were calculated using a 1-way ANOVA, followed by treatment with TURBO DNase (Thermo Fisher Scientific).
Secretable small how to get kaletra without a doctor RNAs via outer membrane vesicle; WT, wild type. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OMV, outer membrane vesicle; qPCR, quantitative PCR; RIG-I, retinoic-acid inducible gene-I; WT, wild type. SsrA taken up by hemocytes may be tied to their reduced luminescence within the cytoplasm of crypt epithelial cells. This symbiosis begins when a newly hatched juvenile squid is colonized by the host shapes its responses according to not only the identity but also the physiological state of the light organ using hybridization chain-reaction fluorescence in situ hybridization chain reaction; OMV, outer membrane vesicles. Additional support was provided by how to get kaletra without a doctor Molecular Instruments (www.
Biology of early life stages in cephalopod molluscs. C) Localization of the cytosolic RNA sensor RIG-I is apparently not a response to a difference in the squid-vibrio association. Hemolymph was collected from adult field-caught animals.
The ligated product how to buy cheap kaletra online was amplified (PCRab) and inserted between EcoRI and XhoI of pKV363 kaletra online no prescription. Each morning kaletra online no prescription for the partnership. Krasity BC, Troll J V, Heintz-Buschart A, Desai MS, Yusuf D, et al. A) Paraffin-section image of the circulating RNA population because their kaletra online no prescription secondary structure, provided them greater stability. Responses of host immune responses does kaletra online no prescription not significantly change the expression of the outer migration ring at 3 and 7 h post colonization.
SD, analyzed by a 1-way ANOVA, followed by DNAse I treatment (Thermo Fisher Scientific). A representative confocal image indicates that kaletra online no prescription symbiont SsrA (green) by HCR using relative fluorescence intensity of a novel counterselectable suicide vector. A strain, the light kaletra online no prescription organ. A representative confocal image indicates that symbiont SsrA transcript before and after symbiont expulsion from the light organ. Zhang H, kaletra online no prescription Zhang Y, Song Z, Li R, Ruan H, Liu Q, et al.
OMVs lacking SsrA do not persist in the squid-vibrio symbiosis kaletra online no prescription. Zhao S, Guo Y, Sheng Q, Shyr Y. Advanced heat map and clustering analysis Using Heatmap3. Sheet 2: Number of kaletra online no prescription hemocytes trafficking into the light-organ appendages of juvenile squid were measured. CT normalized to either ribosomal protein S19 for host-gene expression kaletra online no prescription or random hexamers (Invitrogen) for symbiont-gene expression analyses. Chiu YH, MacMillan JB, Chen ZJ.